Density Gradient Media
Sigma Diagnostics™ Histopaque™-1077 Cell Separation Medium
Facilitates recovery of large numbers of viable lymphocytes and other mononuclear cells from small volumes of whole blood. Sigma-Aldrich™ Histopaque™-1077 is suitable for human lymphocyte antigen (HLA) typing and as the initial isolation step prior to enumeration of T, B, and 'null' lymphocytes.
Sigma Diagnostics™ Accuspin™ Histopaque™-1077 System
Employs centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier (frit). Sigma-Aldrich™ ACCUSPIN™ - System-Histopaque™ -1077 System allows the addition of anticoagulated whole blood without risk of mixing with the separation medium.
Sigma Diagnostics™ Histopaque™-1083 Solution
Facilitates recovery of viable mononuclear cells from rats, mice, and other small mammals. Sigma-Aldrich™ Histopaque™-1083 is for R&D use only, not for drug, household, or other uses.
Sigma Aldrich Fine Chemicals Biosciences OptiPrep Density Gradient Medium | 92339-11-2 | MFCD00867965 | 250 mL
OptiPrep Density Gradient Medium | 92339-11-2 | MFCD00867965 | 250 mL
Cosmo Bio Usa Inc Nycodenz AG, 500G
Nycodenz is a universal density gradient medium provided as an non-ionic, non-toxic powder that is freely soluble in water. Solutions up to 80% (v/w) with a density of 1.426 g/ml can be prepared. It is a tri-iodinated derivative of benzoic acid with three aliphatic hydrophilic side chains. Nycodenz is the ideal medium for:Mammalian and non-mammalian cellsSubcellular organellesOrganelles from nonmammalian sourcesSubcellular membranesProtein and protein complexes
STEMCELL Technologies Lymphoprep™, Size: 250 mL
Reliably isolate mononuclear cells from peripheral blood, cord blood, or bone marrow with Lymphoprep™—a cost-effective alternative to Ficoll-Paque™. Use this density gradient medium for rapid, simple, and reliable cell isolation from most blood samples obtained from normal individuals and patients. You can substitute Lymphoprep™ for Ficoll-Paque™ without changing your existing protocols and achieve similar cell purity and recovery rates. This medium is fully compatible with both SepMate™ and RosetteSep™. Formulated with sodium diatrizoate (9.1% w/v) and polysaccharide (5.7% w/v), Lymphoprep™ has a density of 1.077 g/ml. Visit STEMCELL.com/lymphoprep for more information and to see the performance data.
Cosmo Bio Usa Inc OptiPrep™ 1x250ml
OptiPrep™ is a sterile and endotoxin tested multi-purpose medium based on iodixanol. Unlike any of these other gradient media, iodixanol (as an X-ray imaging agent) has been clinically tested and is manufactured under strict and EU cGMP compliance.OptiPrep™ specifications:Iodixanol: 60% (w/v)Density: 1.320 ± 0.001 g/mlOsmolality: approx. 170 mOsmEndotoxins: < 1.0 EU/ml
Sigma Aldrich Fine Chemicals Biosciences Percoll(R) pH 8.5-9.5 (25 C), suitable for cell culture | MFCD00131830 | 500ML
Percoll(R) pH 8.5-9.5 (25 C), suitable for cell culture | MFCD00131830 | 500ML
Cosmo Bio Usa Inc Lymphoprep™ 4x250 ml
Lymphoprep™ is a ready-made, sterile, and endotoxin tested solution that has been used to isolate mononuclear cells for more than 35 years. Mononuclear cells (monocytes and lymphocytes) have a lower buoyant density than erythrocytes and polymorphonuclear (PMN) leukocytes (granulocytes). The vast majority of mononuclear cells have densities below 1.077 g/ml. These cells can therefore be isolated by centrifugation on an iso-osmotic medium with a density of 1.077 g/ml. Mononuclear cells are therefore retained at the sample/medium interface while erythrocytes and PMNs sediment through the medium.This method is rapid, simple, and reliable and gives excellent results with blood samples from normal individuals and patients. Lymphoprep™ specifications:Sodium diatrizoate: 9.1% (w/v) Polysaccharide: 5.7% (w/v) Density: 1.077 ± 0.001 g/mL; Osmolality: 280 ± 15 mOsm; Endotoxins: <1.0 EU/mL
Supply Solutions Histopaque-1077, 6x100mL
Sterile-filtered, density: 1.077 g/mL, Histopaque-1077 is a sterile, endotoxin tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.077g/mL. This ready-to-use medium facilitates rapid recovery of viable lymphocytes and other mononuclear cells from small volumes of whole blood.
Sigma Aldrich Fine Chemicals Biosciences Percoll, MFCD00131830, 100mL
pH 8.5-9.5 (25 °C), suitable for cell culture, Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP).
Sigma Aldrich Fine Chemicals Biosciences FicollR Paque Premium Cyti
Ficoll-Paque PREMIUM products are a range of sterile ready-to-use density gradient media for the preparation of mononuclear cells.Ficoll-Paque PREMIUM products are based on Ficoll-Paque PLUS (see Ficoll-Paque PLUS) which has been used for more than 30 yr as a sterile density medium for the isolation of high yields of mononuclear cells from bone marrow peripheral blood and cord blood. All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management System certified to ISO 134852003.