Density Gradient Media
Sigma Diagnostics™ Histopaque™-1077 Cell Separation Medium
Facilitates recovery of large numbers of viable lymphocytes and other mononuclear cells from small volumes of whole blood. Sigma-Aldrich™ Histopaque™-1077 is suitable for human lymphocyte antigen (HLA) typing and as the initial isolation step prior to enumeration of T, B, and 'null' lymphocytes.
Sigma Diagnostics™ Histopaque™-1083 Solution
Facilitates recovery of viable mononuclear cells from rats, mice, and other small mammals. Sigma-Aldrich™ Histopaque™-1083 is for R&D use only, not for drug, household, or other uses.
Sigma Diagnostics™ Accuspin™ Histopaque™-1077 System
Employs centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier (frit). Sigma-Aldrich™ ACCUSPIN™ - System-Histopaque™ -1077 System allows the addition of anticoagulated whole blood without risk of mixing with the separation medium.
STEMCELL Technologies Lymphoprep™, Size: 250 mL
Reliably isolate mononuclear cells from peripheral blood, cord blood, or bone marrow with Lymphoprep™—a cost-effective alternative to Ficoll-Paque™. Use this density gradient medium for rapid, simple, and reliable cell isolation from most blood samples obtained from normal individuals and patients. You can substitute Lymphoprep™ for Ficoll-Paque™ without changing your existing protocols and achieve similar cell purity and recovery rates. This medium is fully compatible with both SepMate™ and RosetteSep™. Formulated with sodium diatrizoate (9.1% w/v) and polysaccharide (5.7% w/v), Lymphoprep™ has a density of 1.077 g/ml. Visit STEMCELL.com/lymphoprep for more information and to see the performance data.
Cosmo Bio Usa Inc OptiPrep™ 1x250ml
OptiPrep™ is a sterile and endotoxin tested multi-purpose medium based on iodixanol. Unlike any of these other gradient media, iodixanol (as an X-ray imaging agent) has been clinically tested and is manufactured under strict and EU cGMP compliance.OptiPrep™ specifications:Iodixanol: 60% (w/v)Density: 1.320 ± 0.001 g/mlOsmolality: approx. 170 mOsmEndotoxins: < 1.0 EU/ml
Sigma Aldrich Fine Chemicals Biosciences OptiPrep Density Gradient Medium | 92339-11-2 | MFCD00867965 | 250 mL
OptiPrep Density Gradient Medium | 92339-11-2 | MFCD00867965 | 250 mL
Sigma Aldrich Fine Chemicals Biosciences Histopaque-1119, 6 x 100 mL
A solution containing polysucrose and sodium diatrizoate, adjusted to a density of 1.119 g/mL. Combined with Histopaque-1077, it permits the separation of mononuclear cells and granulocytes.
Cosmo Bio Usa Inc Nycodenz AG, 500G
Nycodenz is a universal density gradient medium provided as an non-ionic, non-toxic powder that is freely soluble in water. Solutions up to 80% (v/w) with a density of 1.426 g/ml can be prepared. It is a tri-iodinated derivative of benzoic acid with three aliphatic hydrophilic side chains. Nycodenz is the ideal medium for:Mammalian and non-mammalian cellsSubcellular organellesOrganelles from nonmammalian sourcesSubcellular membranesProtein and protein complexes
FUJIFILM BIOSCIENCES INC ISolate Stock Solution, 100 mL
ISolate Stock is a 90% density gradient medium used for single layer sperm separation or as the lower layer of a customized gradient developed by the end user based on the semen quality. As one of the most cited density gradient sperm separation media, ISolate Stock is the ideal medium for laboratories that prefer single layer separation or more control over their sperm yields.
Sigma Aldrich Fine Chemicals Biosciences ficollR Paque PREMIUM 1.07
ficoll-Paque PREMIUM products are a range of sterile ready-to-use density gradient media for the preparation of mononuclear cells for use in cell therapy applications.
Cosmo Bio Usa Inc Lymphoprep™ 4x250 ml
Lymphoprep™ is a ready-made, sterile, and endotoxin tested solution that has been used to isolate mononuclear cells for more than 35 years. Mononuclear cells (monocytes and lymphocytes) have a lower buoyant density than erythrocytes and polymorphonuclear (PMN) leukocytes (granulocytes). The vast majority of mononuclear cells have densities below 1.077 g/ml. These cells can therefore be isolated by centrifugation on an iso-osmotic medium with a density of 1.077 g/ml. Mononuclear cells are therefore retained at the sample/medium interface while erythrocytes and PMNs sediment through the medium.This method is rapid, simple, and reliable and gives excellent results with blood samples from normal individuals and patients. Lymphoprep™ specifications:Sodium diatrizoate: 9.1% (w/v) Polysaccharide: 5.7% (w/v) Density: 1.077 ± 0.001 g/mL; Osmolality: 280 ± 15 mOsm; Endotoxins: <1.0 EU/mL
Sigma Aldrich Fine Chemicals Biosciences Percoll(R) pH 8.5-9.5 (25 C), suitable for cell culture | MFCD00131830 | 500ML
Percoll(R) pH 8.5-9.5 (25 C), suitable for cell culture | MFCD00131830 | 500ML
Supply Solutions Histopaque-1077, 6x100mL
Sterile-filtered, density: 1.077 g/mL, Histopaque-1077 is a sterile, endotoxin tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.077g/mL. This ready-to-use medium facilitates rapid recovery of viable lymphocytes and other mononuclear cells from small volumes of whole blood.
